Mar 25, 2019

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by: adminbiotec

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Categories: Invitrogen

Lose pain, gain RNA integrity: New Qubit products

Getting an accurate RNA integrity number (RIN) value can be complicated and downright painful. But now with the new Invitrogen™ Qubit™ 4 Fluorometer, combined with the new Invitrogen™ Qubit™ RNA IQ Assay Kit, accurate RNA assessment is simple and easy to obtain.

No more gels, no tedious pipetting, results in seconds—just add Qubit RNA IQ working solution to your sample and read on the Qubit 4 Fluorometer.

Introducing the breakthrough Invitrogen Qubit RNA IQ Assay, developed to quickly assess the quality and integrity of an RNA sample. This assay allows assessment of RNA quality at a lower cost and with an easier, simpler, and faster workflow than other solutions currently on the market.

The Invitrogen Qubit 4 Fluorometer and Qubit RNA IQ Assay offer the following features:

  • Convenience—use as little as 1 μL of sample
  • Ease of use—simply add RNA sample to the RNA IQ working solution, then measure with the Qubit 4 Fluorometer
  • Speed—obtain accurate measurement of RNA degradation in ~4 seconds per sample

How it works

The Qubit RNA IQ Assay utilizes two unique dyes—one that binds to large, intact and/or structured RNA, the other selectively binds to small, degraded RNA (Figure 1). Together they are able to quickly assess the quality and integrity of an RNA sample. To use, simply add your samples to the RNA IQ working solution, then measure on the Qubit 4 Fluorometer.

Results are presented as a total value for the RNA sample integrity and quality or RNA IQ# and as the calculated % of large and small RNA in the sample. The RNA IQ# is based on a scale of 1 to 10 similar to other RNA quality scores, wherein a high IQ# indicates the majority of the sample consists of large and/or structured RNA. Conversely a small IQ# indicates the sample comprises mainly small RNA with limited tertiary structure